Electrogenerated Chemiluminescence (ECL)
While ECL is a lesser known luminescence technique, it is by no means less useful. The ability to generate localized light emission without a light based stimulation source immediately offers numerous analytical advantages. It’s these advantages that make ECL a potent detection methodology. Our group plans to utilize ECL to develop a novel signal amplification strategy. This system is based around a multi-disciplinary approach to redefining the relationship between the luminophore label and the antibody tag. In 2013 our team published the proof of concept for this approach.
Despite promising results, the project required two lines of effort to quantitatively evaluate the amplification capability of the nanospheres. The first would require the synthetic development of an ECL monomer that had well defined performance characteristics. The second would require the development of an analytical setup where light emission could be quantitatively measured. In 2017 our team released our preliminary efforts into both avenues.
Danis, A.S. et al. ChemElectroChem 2017
Future work for this project will involve further refinement of the analytical ECL setup and characterization of our ruthenium monomer’s ECL performance in different coordination environments.
Recent group publications
4. Danis, A.S.; Gordon, J.B.; Potts, K.P.; Stephens, L.I.; Perry, S.C.; Mauzeroll, J. Simultaneous Electrochemical and Emission Monitoring of Electrogenerated Chemiluminescence through Instrument Hyphenation. Analytical Chemistry 2019, 91(3), 2312-2318.
3. Danis, A.S.; Potts, K.P.; Perry, S.C.; Mauzeroll, J. Combined Spectroelectrochemical and Simulated Insights into the Electrogenerated Chemiluminescence Coreactant Mechanism. Analytical Chemistry 2018, 90(12), 7377-7382.
2. Danis, A.; Odette, W.; Perry, S.C.; Sylvain, C.; Sleiman, H.; Mauzeroll, J. Cuvette-Based Electrogenerated Chemiluminescence Detection System for the Assessment of Polymerizable Ruthenium Luminophores. ChemElectroChem 2017, 4(7), 1736-1743.
1. Tefashe, U. M.; Metera, K. L.; Sleiman, H. F.; Mauzeroll, J., Electrogenerated Chemiluminescence of Iridium-Containing ROMP Block Copolymer and Self-Assembled Micelles. Langmuir 2013, 29 (41), 12866-12873.
4. Danis, A.S.; Gordon, J.B.; Potts, K.P.; Stephens, L.I.; Perry, S.C.; Mauzeroll, J. Simultaneous Electrochemical and Emission Monitoring of Electrogenerated Chemiluminescence through Instrument Hyphenation. Analytical Chemistry 2019, 91(3), 2312-2318.
3. Danis, A.S.; Potts, K.P.; Perry, S.C.; Mauzeroll, J. Combined Spectroelectrochemical and Simulated Insights into the Electrogenerated Chemiluminescence Coreactant Mechanism. Analytical Chemistry 2018, 90(12), 7377-7382.
2. Danis, A.; Odette, W.; Perry, S.C.; Sylvain, C.; Sleiman, H.; Mauzeroll, J. Cuvette-Based Electrogenerated Chemiluminescence Detection System for the Assessment of Polymerizable Ruthenium Luminophores. ChemElectroChem 2017, 4(7), 1736-1743.
1. Tefashe, U. M.; Metera, K. L.; Sleiman, H. F.; Mauzeroll, J., Electrogenerated Chemiluminescence of Iridium-Containing ROMP Block Copolymer and Self-Assembled Micelles. Langmuir 2013, 29 (41), 12866-12873.