multidrug resistance
Cancer is a devastating disease and multidrug resistance can seriously undermine chemotherapeutic treatment. One way cancer cells defend themselves is through efflux pumps which can transport chemotherapeutic drugs out of the cell. One such pump studied in our lab is the multidrug resistance-associated protein 1 (MRP1), an ATP-binding cassette transporter. Correlation studies between MRP1 and cancer patient’s prognosis often focus on MRP1 expression and not activity.
Our goal is to study the relationship between MRP1 expression and activity and to ultimately track multidrug resistance in cancer patients. We have developed a bioanalytical method for noninvasive evaluation of MRP1 activity on a single cell level. This method relies on the unique properties of scanning electrochemical microscopy (SECM) to measure cell efflux and determine MRP1 activity with high spatial resolution. Flow cytometry can be used to determine MRP1 expression. This project includes the development of patterning strategies for SECM studies, in collaboration with the National Research Council Canada.
Our goal is to study the relationship between MRP1 expression and activity and to ultimately track multidrug resistance in cancer patients. We have developed a bioanalytical method for noninvasive evaluation of MRP1 activity on a single cell level. This method relies on the unique properties of scanning electrochemical microscopy (SECM) to measure cell efflux and determine MRP1 activity with high spatial resolution. Flow cytometry can be used to determine MRP1 expression. This project includes the development of patterning strategies for SECM studies, in collaboration with the National Research Council Canada.
Recent group publications
2. Polcari, D.; Hernandez-Castro, J.A.; Li, K.; Geissler, M.; Mauzeroll, J. Determination of the Relationship between Expression and Functional Activity of Multidrug Resistance-Associated Protein 1 using Scanning Electrochemical Microscopy. Analytical Chemistry 2017, 89(17), 8988-8994.
1. Kuss, S.; Polcari, D.; Geissler, M.; Brassard, D.; Mauzeroll, J., Assessment of multidrug resistance on cell coculture patterns using scanning electrochemical microscopy. Proceedings of the National Academy of Sciences 2013, 110 (23), 9249-9254.
2. Polcari, D.; Hernandez-Castro, J.A.; Li, K.; Geissler, M.; Mauzeroll, J. Determination of the Relationship between Expression and Functional Activity of Multidrug Resistance-Associated Protein 1 using Scanning Electrochemical Microscopy. Analytical Chemistry 2017, 89(17), 8988-8994.
1. Kuss, S.; Polcari, D.; Geissler, M.; Brassard, D.; Mauzeroll, J., Assessment of multidrug resistance on cell coculture patterns using scanning electrochemical microscopy. Proceedings of the National Academy of Sciences 2013, 110 (23), 9249-9254.