biosensors
The field of biosensors has been growing since the development of the widely used commercial glucose oxidase sensors. By definition, a biosensor is an analytical device where the sensing element consists of a biological component, typically an enzyme. Currently, there are many challenges in the fabrication of these sensors including poor thermal stability and pH sensitivity of the enzymes. As a result there is a limited pool of enzymes currently in use for these types of devices. Discovering more possible enzymes for biosensor development is one way to unlock a plethora of possible applications.
Our group uses a methodology developed in-house for microelectrode fabrication to devise and optimize the design of the biosensors. The advantage of a microelectrode-based technique is spatial resolution for systems of interest. Since enzymatic biosensors are favorable due to their selectivity and sensitivity to specific analytes, they are highly suitable for use in vitro. These concepts have been applied by our group to successfully fabricate and test ᴅ-serine sensing enzymatic biosensors.
Our group uses a methodology developed in-house for microelectrode fabrication to devise and optimize the design of the biosensors. The advantage of a microelectrode-based technique is spatial resolution for systems of interest. Since enzymatic biosensors are favorable due to their selectivity and sensitivity to specific analytes, they are highly suitable for use in vitro. These concepts have been applied by our group to successfully fabricate and test ᴅ-serine sensing enzymatic biosensors.
Polcari, D. et al. Anal. Chem., 2014, 86 (7), pp 3501–3507
As improvements in the performance of biosensors to meet specific needs is a progressing area, future work includes optimizing biosensor design. Another area of interest is expanding the pool of enzymes used in making these biosensors to include a larger number of enzymes.
Recent group publications
- Moussa, S., Van Horn, M., Shah, A., Pollegioni, L., Thibodeaux, C.; Ruthazer, E., Mauzeroll, J. A Miniaturized Enzymatic Biosensor For Detection of Sensory-Evoked ᴅ-serine Release in the Brain, Journal of the Electrochemical Society 2021, 168, 025502 (Editor’s Choice)
- Polcari, D.; Perry, S.C.; Pollegioni, L.; Geissler, M.; Mauzeroll, J. Localized Detection of D-Serine Using an Enzymatic Amperometric Biosensor and Scanning Electrochemical Microscopy. ChemElectroChem 2017, 4, 920-926.
- Polcari, D.; Kwan, A.; Van Horn, M. R.; Danis, L.; Pollegioni, L.; Ruthazer, E. S.; Mauzeroll, J., Disk-Shaped Amperometric Enzymatic Biosensor for In Vivo Detection of D-serine. Analytical Chemistry 2014, 86 (7), 3501-3507